Exploration of new effective fibrinolytic agent from Alcaligenes aquatilis PJS_1 from polluted soil

Aim: To isolate and identify Alcaligenes aquatilis PJS_1 from slaughter house soil samples for production of enzymatic fibrinolytic agent productionMethodology: Fibrinolytic enzyme producing bacterium was isolated from slaughter house soil samples and identified by biochemical tests and 16S rRNA sequencing. The fibrinolytic enzyme production media was optimized by various factors like energy sources, pH and temperature. Bioreactor used in the experiment was designed with suitable parameters for effective production and purification is by gel filtration chromatography. Blood clotting assay was performed to determine its anticoagulant property. Results: The isolated enzyme producing bacterium was identified as Alcaligenes aquatilis PJS_1. The medium with fructose and urea at pH 7.0 was found to have optimum production when incubated for 24 hr at 37ºC. The crude enzyme was purified by acetone precipitation followed by gel filtration chromatography. The enzyme showed a final specific activity of 629.32 Umg-1 with of 88.24% yield Interpretation: The present study provides information that the enzyme produced by Alcaligenes aquatilis PJS_1 acts as an effective fibrinolytic agent

Sequestration and purification of essential enzyme from Pleurotus florida compost waste and its application studies

Spent oyster mushroom (Pleurotus florida) compost as tremendous source for isolation of industrial significantenzymes such as amylase, protease, and cellulase. This study was conducted to achieve efficient extraction oflignocellulolytic enzymes amylase (EC 3.2.1.1), cellulase (EC 3.2.1.4), and protease (EC 3.4.21.14) from spent oystermushroom (P. florida) compost waste. Optimal enzyme recovery was achieved when spent oyster mushroom compostwastes and concentrated by acetone precipitation. The purification was performed by column chromatography. Theenzymes such as cellulase, amylase, and protease released from oyster mushroom (P. florida) compost waste wereshowed activities of 15.78 U/ml, 3.42 U/ml, and 0.042 U/ml, respectively. These were utilized in various industrialand environmental applications such as starch processing in potato waste from food industry using amylase andbiotreatment of cotton waste using cellulase.

Isolation and molecular identification of Mycoplasma equigenitalium from equine genital tracts in northern India

Although Mycoplasma equigenitalium has been implicated in equine reproductive problems, its prevalence is largely unexplored due to the lack of specific diagnostic tests. To address this limitation, the authors developed and optimized species-specific primer pairs that target M. eguigenitalium rpoB [RNA polymerase B subunit] gene sequences. The specificity of the PCR assay developed in this study was determined using 12 field isolates including the type strain of M. equigenitalium and other Mycoplasma species. In the field study, a total of 122 mare and stallion samples comprising of 50 clinical and 72 random samples were subjected to speciesspecific PCR assay to detect M. equigenitalium in equine genital tracts. Mycoplasma equigenitalium [MEG] species-specific PCR detected 22.13% positive samples; however, only 9.01% of the samples were found to be positive using the conventional culture technique. The PCR established in this study could be used for rapid, specific and accurate diagnosis of M. equigenitalium strains. To the authors' knowledge, this is the first report addressing the development and evaluation of species-specific PCR to detect M. equigenitalium